Write Channel Model for Bit-Patterned Media Recording

We propose a new write channel model for bit-patterned media recording that reflects the data dependence of write synchronization errors. It is shown that this model accommodates both substitution-like errors and insertion-deletion errors whose statistics are determined by an underlying channel state process. We study information theoretic properties of the write channel model, including the capacity, symmetric information rate, Markov-1 rate and the zero-error capacity.Comment: 11 pages, 12 figures, journa

Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins

Fluorescent fusion proteins are widely used to study protein localization and interaction dynamics in living cells. However, to fully characterize proteins and to understand their function it is crucial to determine biochemical characteristics such as enzymatic activity and binding specificity. Here we demonstrate an easy, reliable and versatile medium/high-throughput method to study biochemical and functional characteristics of fluorescent fusion proteins. Using a new system based on 96-well micro plates comprising an immobilized GFP-binding protein (GFP-mulitTrap), we performed fast and efficient one-step purification of different GFP- and YFP-fusion proteins from crude cell lysate. After immobilization we determined highly reproducible binding ratios of cellular expressed GFP-fusion proteins to histone-tail peptides, DNA or selected RFP-fusion proteins. In particular, we found Cbx1 preferentially binding to di-and trimethylated H3K9 that is abolished by phosphorylation of the adjacent serine. DNA binding assays showed, that the MBD domain of MeCP2 discriminates between fully methylated over unmethylated DNA and protein-protein interactions studies demonstrate, that the PBD domain of Dnmt1 is essential for binding to PCNA. Moreover, using an ELISA-based approach, we detected endogenous PCNA and histone H3 bound at GFP-fusions. In addition, we quantified the level of H3K4me2 on nucleosomes containing different histone variants. In summary, we present an innovative medium/high-throughput approach to analyse binding specificities of fluroescently labeled fusion proteins and to detect endogenous interacting factors in a fast and reliable manner in vitro

California Cities Gang Prevention Network Bulletin (Bulletin 3)

NCCD and the National League of Cities' Institute for Youth, Education, and Families (YEF Institute) present the bulletin for the California Cities Gang Prevention Network. This initiative creates a network of major California cities to combat gang violence and victimization

Gaussian Belief Propagation Based Multiuser Detection

In this work, we present a novel construction for solving the linear multiuser detection problem using the Gaussian Belief Propagation algorithm. Our algorithm yields an efficient, iterative and distributed implementation of the MMSE detector. We compare our algorithm's performance to a recent result and show an improved memory consumption, reduced computation steps and a reduction in the number of sent messages. We prove that recent work by Montanari et al. is an instance of our general algorithm, providing new convergence results for both algorithms.Comment: 6 pages, 1 figures, appeared in the 2008 IEEE International Symposium on Information Theory, Toronto, July 200